Implemented kill switches: 1
Kill Switch 1
Class
# Format: # Write how you would classify them.
Toxins
| Name | Number | Comment |
|---|---|---|
| Bak | inducer of apoptosis |
Antagonists
| Name | Number | Comment |
|---|---|---|
| Stop codon | upon integration into the genome the stop codon will stop the translation in front of Bak | |
| SV40PA | upon integration into the genome the polyadenylation site will ensure the stop of translation |
Topology
Parts
| Name | Number | Comment |
|---|---|---|
| CMV promoter | inducible by tetracycline | |
| attB | bacterial attachment site for integration | |
| attP | phage attachment site for integration | |
| SV40PA | downstream ployadenylation site | |
| Phic31o | Integrase for integration | |
| CMV | sucrose inducible promoter |